mouse anti il10rb antibody  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Single-cell and spatial profiling reveal an IL-10–associated iCAF–M2 macrophage communication axis in high-grade serous ovarian cancer ascites

doi: 10.3389/fimmu.2026.1790912

Figure Lengend Snippet: IL10 blockade with MK-1966 suppresses tumor growth and decreases IL10 pathway activity in humanized PDX models. (A) Schematic of humanized ovarian cancer PDX model generation and MK-1966 treatment timeline. (B) Representative tumor images demonstrating visibly smaller tumors in MK-1966–treated mice. (C) Tumor growth curves showing significantly reduced tumor volume following MK-1966 treatment (n = 5, p < 0.001). (D) Final tumor weight demonstrating significantly lower tumor mass in treated mice (n = 5, p < 0.001). (E) Representative IHC staining showing decreased CD206, Ki67, IL-10, and IL-10RA expression in MK-1966 tumors versus controls. (F) Quantitative IHC scoring confirming significant reductions in CD206 and Ki67 ( p < 0.01) and IL-10 and IL-10RA ( p < 0.05) following treatment. N = 3 (G) Representative multiplex immunofluorescence staining of DAPI (blue), EpCAM (green), and Ki67 (red) demonstrating co-localization of Ki67-positive proliferating cells within EpCAM-positive tumor cells. MK-1966 treatment markedly reduced EpCAM + /Ki67 + double-positive tumor cells compared with controls. (H) Quantitative analysis of EpCAM + /Ki67 + relative fluorescence intensity demonstrating significantly decreased tumor-cell proliferative activity following MK-1966 treatment (** p< 0.01). * represent p-value < 0.05; *** represent p-value < 0.001.

Article Snippet: Once tumors reached ~100 mm3, animals were randomized into: Control group (vehicle) and MK-1966 treatment group (anti-IL-10 monoclonal antibody; MCE, HY-P991257).

Techniques: Activity Assay, Immunohistochemistry, Expressing, Multiplex Assay, Immunofluorescence, Staining, Fluorescence

Journal: Bioactive Materials

Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

doi: 10.1016/j.bioactmat.2026.03.025

Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: Materials Today Bio

Article Title: Spatiotemporal piezoelectric microspheres for wireless endometrial repair with improved pregnancy outcomes

doi: 10.1016/j.mtbio.2026.102915

Figure Lengend Snippet: Characterization of the uterus tissue with SPCM treatment. a, α-SMA staining gauged smooth-muscle thickness. Scale bars, 100 μm. (The white line indicates smooth muscle thickness). b-c, VEGF/MVD immunofluorescence (200 μm) mapped blood vessels. Scale bars, 200 μm. d-e, IL-10 and IL-6 staining quantified local inflammation. Scale bars, 200 μm. g-i, Quantitative analysis of the molecular expression levels mentioned above in the uterus tissues. ∗ P < 0.05 for One Way ANOVA.

Article Snippet: After dewaxing and rehydration, tissue sections were incubated in 3% H 2 O 2 and blocking solution, followed by primary antibodies against α-SMA (proteintech, 14395-1-AP, diluted 1:200, China), VEGF (proteintech, 19003-1-AP, diluted 1:200, China), MVD (proteintech, 15331-1-AP, diluted 1:200, China), IL-10 (proteintech, 60269-1-Ig, diluted 1:200, China), IL-6 (proteintech, 26404-1-AP, diluted 1:200, China) and Ki67 (proteintech, 28074-1-AP, diluted 1:400, China) at 4 °C overnight.

Techniques: Staining, Immunofluorescence, Expressing

Journal: Molecular & Cellular Proteomics : MCP

Article Title: An Integrative Proteotranscriptomics Approach Reveals New ADAM9 Substrates and Downstream Pathways

doi: 10.1016/j.mcpro.2026.101538

Figure Lengend Snippet: Identification of IGSF8 and IL10RB as new ADAM9 substrates. HCT116 cells were transfected with the indicated siRNA or expression constructs, and Western blotting was performed for conditioned media (CM) or cell lysates (CLs) using antibodies recognizing the ectodomain of IGSF8 ( A , B ) or IL10RB ( C , D ). Three biological replicates are shown in ( A , C ); unpaired two-tailed t test was performed. ADAM, A Disintegrin And Metalloproteinase; IGSF8, immunoglobulin superfamily member 8; IL10RB, interleukin 10 receptor subunit beta.

Article Snippet: Antibodies used in this study include rabbit anti-ADAM9 (CST, 4151, 1:1000 dilution), mouse anti-ALCAM (Santa Cruz, 74558, 1:200 dilution), mouse anti–interleukin 10 receptor subunit beta (IL10RB) (R&D Systems, MAB874, 5 μg/ml), rabbit anti–Forkhead Box O3 (FOXO3) (CST, 2497, 1:1000 dilution for Western blotting, and 1:100 dilution for immunocytochemistry [ICC]), rabbit anti–immunoglobulin superfamily member 8 (IGSF8) (Sigma, 011917, 1:500 dilution), horseradish peroxidase (HRP)–conjugated rabbit anti-mouse (CST, 7076, 1:2000 dilution), HRP-conjugated goat anti-rabbit (CST, 7074; 1:2000 dilution), HRP-conjugated mouse anti-β-actin antibody (CST, 12262, 1:2000 dilution), and Alexa Fluor 594–conjugated goat anti-rabbit antibody (Thermo Fisher, A-11012, 1:1000 dilution for ICC).

Techniques: Transfection, Expressing, Construct, Western Blot, Two Tailed Test